Sterilization

Microfluidic PimCell® Dishes need end user sterilization and should be sterilized by washing in 70% ethanol for 15 minutes followed by 3 times washing with a large volume of deionized water.

Reagents

  • Deionized (DI) water
  • Ethanol: 70% in sterile DI water

Equipment & Materials

·       Biosafety cabinet

·       Gloves

·       Tweezers

·       Washing reservoir

·       Sterile napkin

·       Petri dish

Microfluidic PimCell® Dish sterilization procedure

  1. Transfer a PimCell® Dish to a sterile laminar-flow biosafety cabinet
  2. In the biosafety cabinet, remove the lid of the PimCell® Dish using clean gloves and transfer the dish with its lid to a washing  reservoir
  3. Rinse inserts in 70% ethanol for 15 minutes followed by 3 times washing with a large volume of deionized water
  4. Transfer the PimCell® Dish with its lid to a sterile napkin using tweezers to dry the glass cover slip of the dish
  5. Gently remove rests of water with a sterile pipette
  6. Transfer the PimCell® Dish with its lid to a sterile Petri dish using tweezers
  7. Place under UV-light for 15 minutes in the biosafety cabinet under sterile conditions

The sterilized devices can be stored at room temperature 23°C for 24 hours.